NGS for somatic SNVs and indels – tumour with germline subtraction analysis
EQA for quality of NGS for somatic SNVs and indels <50bp (FFPE). For NGS requiring a matched normal control germline sample (single gene, panel testing, whole exome sequencing or whole genome sequencing). Pilot EQA.
Overview
This EQA scheme is NOT suitable for somatic NGS workflows
that DO NOT require a matched normal control sample. Please see our DNA
SEQUENCING – NGS (Somatic – no matched germline sample) EQA scheme.
At a glance
Product Code:
03-NGSS-PLMS-27
Target/Genes:
Participating laboratories can use any NGS strategy to
participate (single gene, panel testing, whole exome sequencing or whole genome
sequencing). This EQA scheme includes a matched germline normal sample for laboratories that use germline variant subtraction.
The scheme will assess all single nucleotide variants and indels 50bp or less in size.
Participants will be asked what NGS strategy they are likely to use in participation (single gene, panel testing, whole exome sequencing or whole genome sequencing or both) at the point of purchase. Please contact us at office@emqn.org if your needs change or with any queries.
Technology:
NGS, Whole exome sequencing, Whole genome sequencing
Sample Type:
DNA (in TE Buffer)
Submit Results In:
Not applicable
Accredited:
No
Key Dates
01/03/2027
Sep-27
September - November 2027
Sample Details
One mock clinical case with matching tumour DNA and germline DNA samples will be provided. Approximately 1-2µg DNA per sample is provided depending on testing strategy. Complete information will be available in the scheme instructions. Laboratories will be contacted prior to sample dispatch to obtain testing strategy for sample requirements.
EQA Results
Participants will receive a comprehensive individual data quality report and a variant consensus analysis report for each submission. A comprehensive summary scheme report is provided including additional information from the cohort of participants including geographical spread, methodologies employed, common errors, learning points and scheme statistics allowing participants to benchmark their results.
Each laboratory will also receive a single certificate listing all EQAs participated in with performance result for each year.
Participation Information
Participants are expected to test samples according to their routine NGS strategy, to submit VCF, BED, FASTQ and BAM files for analysis.
Laboratories can submit up to three different SNV / indel data sets per participation, derived from different analyses of the EQA sample (e.g. different panels or methodologies).
No restrictions on number of participants. Open to laboratories from ALL countries (note: some restrictions may apply due to sanctions, delivery network exceptions, restrictions on importation etc).
This EQA is provided in collaboration with GenQA.
Send your enquiry by submitting the form
Closes in 112 days
From £1195.00
Buying is completed on the EMQN portal and needs a free EMQN account, which takes a few minutes to set up.
Questions about this scheme?
Contact our team
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